Rooms
The Department of Biochemistry I has four molecular biology and biochemistry laboratories of biosafety level 1, two cell culture laboratories of biosafety level 1, three electrophysiology laboratories and one microcopy laboratory.
Special Equipment
Electrophysiology
6 setups for two-electrode voltage clamp recordings of oocytes
1 automated high-throughput system for two-electrode voltage clamp recordings of oocytes Multichannel Systems Roboocyte
3 injection setups for microinjection into oocytes
1 patch clamp setup with perfusion system for fast ligand application
Semi-automatic patch clamp setup PatchMAX100A with pressure controller ez-gSEAL for recordings from cultured cells and oocytes
Property of the Research Department of Neuroscience
Confocal Microscopy
1 confocal laser-scanning microscope Leica TCS SP2 AOBS with an optional aerated incubation chamber for long-term experiments with living cells
Excitation wavelengths: 458 nm, 476 nm, 488 nm, 514 nm, 543 nm, 594 nm, 633 nm
DNA Sequencing
1 capillary sequencer Applied Biosystems 3130xl Genetic Analyzer
DNA sequencing service for all faculties of the Ruhr University Bochum
Molecular Biology
1 real-time PCR machine Roche LightCycler
Protein Purification
- FPLC system Pharmacia HiLoad
Centrifuges
2 high speed centrifuges Sorvall RC28S
2 high speed centrifuges Sorvall RC5B
1 ultracentrifuge Sorvall Discovery 90
1 ultracentrifuge Sorvall Discovery M120
1 ultracentrifuge Sorvall OTD65B
1 ultracentrifuge Sorvall OTD Combi