Total Internal Reflection (TIRF)/Epi Fluorescence Microscope Olympus IXplore TIRF
The Olympus IX83 inverted microscope is equipped with a TIRF module and enables both simultaneous and sequential use of TIRF, wide-field fluorescence, and transmitted light differential interference contrast (DIC) method. The system allows for excitation and detection of up to two fluorophors simultaneously, via projection of each fluorophor’s signal onto one half of the chip of an sCMOS camera. Alternatively, single-color TIRF can be performed using the bypass modus of the optosplit. Access to the instrument is open for any member of the Ruhr University Bochum after initial registration and training.
Technische Ausstattung
- LED light source : ~345 nm ~385 nm, ~465 nm, ~505 … 600 nm (selectable by bandpass filter), ~625 nm
- Laser lines 488 nm, 561 nm and 640 nm, angles adjustable independently from each other
- Motorized stage for multiple position imaging
- Temperature control with incubation chamber, CO2 mixer.
- ORCA-FLASH4.0V3 digital sCMOS camera
- 20x, 40x NA 1.35, 60x NA 1.2, 100x NA 1.5 oil objectives, the 40x and 100x objectives are optimized for UV transmission and TIRF, respectively.
Dr. Sebastian Neumann