Confocal Laser Scanning Microscope Leica TCS SP8 WLL SMD-FLIM-FCS
The Leica TCS SP8 FLIM FCS system enables quantitative fluorescence intensity and lifetime imaging as well as fluorescence correlation spectroscopy all on the same confocal microscope. Users can seamlessly switch between these advanced fluorescence techniques to analyse fluorophore localisation and molecular dynamics.
Access to the instrument is open for any member of the Ruhr University Bochum after initial registration and training.
Technical Features
- A pulsed white light laser (WLL) provides up to eight fully tunable, excitation lines between 470 and 670 nm simultaneously for maximal spectral flexibility. The white light laser is suitable for all SMD techniques.
- A pulsed 440 nm laser for SMD experiments with dyes that are excited below 470 nm.
- A 405 nm laser and a 442 nm laser (not pulsed).
- Two highly sensitive, low-noise hybrid detectors provide images with high dynamic range and high contrast as well as single photon detection for SMD techniques.
- Images with up to five channels are possible in combination with three built-in photomultipliers.
- A spectral detector enables determination of emission spectra, the white light laser determination of excitation spectra. This allows the full spectral characterisation of the sample.
- A fully motorised stage allows tile scans and z-scans.
- An incubator box with temperature control, gas regulation and humidifier allows long-term experiments on live cells.
Dr. Sebastian Neumann
Dr. Daniel Tapken