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E. Muth-Köhne, J. Terhag, S. Pahl, M. Werner, I. Joshi, and M. Hollmann (2010).
Functional excitatory GABA_A receptors precede ionotropic glutamate receptors in radial glia-like neural stem cells.
Molecular and Cellular Neuroscience 43(2): 209-221.
doi: 10.1016/j.mcn.2009.11.002

The involvement of neurotransmission in neuronal development is a generally accepted concept. Nevertheless, the precise regulation of neurotransmitter receptor expression is still unclear. To investigate the expression profiles of the most important ionotropic neurotransmitter receptors, namely GABA_A receptors (GABA_ARs), NMDA receptors (NMDARs), and AMPA receptors (AMPARs), quantitative RT-PCR, immunoblot analysis and patch clamp studies were performed in in vitro-generated neural stem cells (NSCs). This clearly defined cell line is closely related to radial glia cells, the stem cells in the neonate brain.

We found functional GABA_ARs of the subunit composition α2, β3, and γ1 to be expressed. Unexpectedly, functional ionotropic glutamate receptors were absent. However, NSCs expressed the NMDAR subunits NR2A and NR3A, and the AMPAR subunit GluR4 at the protein level, and GluR3 at the mRNA level.

The overexpression of functional NMDARs in NSCs led to an increased mRNA level of AMPAR subunits, indicating a role in synaptogenesis. Early neuronal markers remained unchanged. These data extend our knowledge about ionotropic neurotransmitter receptor expression during neuronal development and will aid further investigations on activity-dependent neurogenesis.