M. Hollmann, A. O’Shea-Greenfield, S.W. Rogers, and S. Heinemann (1989).
Cloning by functional expression of a member of the glutamate receptor family.
Nature 342(6250): 643-648.
doi: 10.1038/342643a0
We have isolated a complementary DNA clone by screening a rat brain cDNA library for expression of kainate-gated ion channels in Xenopus oocytes. The cDNA encodes a single protein of relative molecular mass (Mr) 99,800 which on expression in oocytes forms a functional ion channel possessing the electrophysiological and pharmacological properties of the kainate subtype of the glutamate receptor family in the mammalian central nervous system.